Chapter 13: Diagnostic tests
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13.9 Lumbar puncture
Essential equipment
- Personal protective equipment
- Antiseptic skin‐cleaning agents, for example chlorhexidine in 70% alcohol or isopropyl alcohol
- Selection of needles and syringes
- Sterile gloves
- Sterile dressing pack
- Lumbar puncture needles of assorted sizes
- Disposable manometer
- Three sterile specimen bottles; these should be labelled 1, 2 and 3
- Plaster dressing
Medicinal products
- Local anaesthetic, for example lidocaine 1%
Pre‐procedure
ActionRationale
- 1.
Introduce yourself to the patient, explain and discuss the procedure with them, and gain their consent to proceed.To ensure that the patient feels at ease, understands the procedure and gives their valid consent (NMC [165], C).
- 2.Patient should empty their bladder and bowels before the procedure.
- 3.Assist patient into the required position on a firm surface. Either lying (Action figure 13.19):Or sitting:
- one pillow under the patient's head
- on side with knees drawn up to the abdomen and clasped by the hands
- support patient to maintain this position.
- patient straddles a straight‐backed chair so that their back is facing the doctor
- patient folds arms on the back of the chair and rests head on them.
- 4.Continue to support and observe the patient throughout the procedure, and explain the procedure.To facilitate psychological and physical wellbeing (Barker and Laia [10], E).
Procedure
- 5.Wash hands with antibacterial detergent. Put on gloves and apron.To reduce the risk of contamination and cross‐infection (Fraise and Bradley [62], E).
- 6.Assist doctor/practitioner as required; doctor/practitioner will proceed to put on a hat and mask, clean hands with antiseptic solution, and put on a sterile apron and gloves.To minimize the risk of cross‐contamination (Gemmell et al. [67], E).
- 7.Clean the skin with the antiseptic cleaning agent.To ensure the removal of skin flora to minimize the risk of infection (Lindsay et al. [126], E).
- 8.Identify the area to be punctured and infiltrate the skin and subcutaneous layers with local anaesthetic.To minimize discomfort from the procedure (Barker and Laia [10], E).
- 9.Introduce a spinal puncture needle below the second lumbar vertebra and into the subarachnoid space.This is below the level of the spinal cord but still within the subarachnoid space (Lindsay et al. [126], E).
- 10.Ensure that the subarachnoid space has been entered and attach the manometer to the spinal needle, if required.
- 11.Obtain the appropriate specimens of CSF (approx. 10 mL) for analysis. Cell count and gram stain can be performed using 1 mL of fluid.To establish a diagnosis (Pagana and Pagana [177], E). The first specimen, which may be blood‐stained due to needle trauma, should go into bottle 1. This will assist the laboratory to differentiate between blood due to procedure trauma and blood due to subarachnoid haemorrhage (Chernecky and Berger [32], E).
- 12.Ensure the specimens are labelled appropriately (as 1, 2 and 3) and sent with the correct forms to the laboratory.
- 13.If intrathecal medication is to be instilled, the drug and dose must be checked and administered safely according to national guidelines.
- 14.Withdraw the spinal needle once specimens have been obtained, appropriate pressure measurements have been taken and intrathecal medication has been administered.To minimize the risks of the procedure (Barker and Laia [10], E).
- 15.When the needle has been withdrawn, apply pressure over the lumbar puncture site using a sterile topical swab.To maintain asepsis and to stop blood and CSF flow (Doherty and Forbes [50], E).
- 16.When all leakage from the puncture site has ceased, apply a plaster dressing.To prevent secondary infection (Barker and Laia [10], E).
Post‐procedure
- 17.Make the patient comfortable. They should lie flat or the head should be tilted slightly downwards. Time to lie flat varies from hospital to hospital, but it is usually around 4 hours if there is no headache.
- 18.Remove equipment and dispose of it as appropriate.To prevent the spread of infection and reduce the risk of needle stick injury (DH [48], C).
- 19.Document the procedure in the patient's records.To ensure timely and accurate record keeping (NMC [165], C).
- 20.Patient should be monitored for the next 24 hours with careful observation of the following:
- leakage from the puncture site
- headache
- backache
- neurological observations and vital signs.
Clear fluid may be a cerebrospinal leak (Barker and Laia [10], E).Headache and backache are not unusual following lumbar puncture and may be due to loss of CSF. E