Chapter 13: Diagnostic tests
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13.11 Cervical uterine smear using liquid‐based cytology
Essential equipment
- Personal protective equipment
- Light source
- Examination couch
- Disposable clean paper couch covers
- Variously sized specula (disposable or reusable)
- Cervix brush
- Liquid‐based cytology (LBC) container
- Black pen for labelling LBC container
- Specimen form and plastic specimen bag
- Container of warm water
- Box of tissue paper
- Clinical waste container
- Trolley
- Lubricant
Optional equipment
- A collecting container for central sterile services (CSS) if reusable instruments are used
Pre‐procedure
ActionRationale
- 1.
Introduce yourself to the patient, explain and discuss the procedure with them, and gain their consent to proceed. Discuss the benefits and limitations of screening and the significance of smear results. Explain the purpose of the procedure and what will occur at each step.To ensure that the patient feels at ease, understands the procedure and gives her valid consent. To ensure that she understands that the procedure involves removing underwear and that the speculum will be inserted into her vagina (NHSCSP [161], C; NMC [165], C). To allow the patient time to ask any questions. E
- 2.Document the woman's clinical and screening history, specifically:
- date of last menstruation
- any abnormal bleeding
- any unusual vaginal discharge
- contraception
- date of last cervical smear
- any abnormal smear results
- any treatment to the cervix.
To ensure a relevant history is recorded (NHSCSP [161], C). - 3.Place all the equipment required for the procedure on the trolley.To facilitate the efficient taking of the sample. E
- 4.Close the room door and/or curtains and ask the woman to remove her underwear.To provide privacy and comfort for the woman. E
Procedure
- 5.Turn the light source on and position it at the end of the examination table.
- 6.Assist the patient into a supine position on the couch, with knees drawn up and legs parted. Keep her as covered as possible.
- 7.Wash hands with antibacterial detergent and running hand‐hot water. Ensure hands are dried with disposable paper hand towels.
- 8.Apply gloves and apron.
- 9.Select the appropriate size of speculum, from small, medium and large, or a long‐bladed narrow speculum if the vagina is long or the cervix is lying posterior. The sterilized speculum can be warmed or cooled using clean tap water.To promote patient comfort and reduce anxiety (NHSCSP [161], C). If removed from a sterilizer, the speculum may need to be cooled down; if cold, it may need to be warmed up to reduce patient discomfort. It must be explained to the patient that the speculum has been sterilized but that the water will not contaminate it. E
- 10.Apply lubricant to the speculum. Part the labia and, holding the speculum blades together sideways, slip the speculum into the vagina.
- 11.When the speculum is half way into the vagina, turn it so that the handle is facing down.To promote patient comfort and reduce contamination of the cervix with lubricants (NHSCSP [161], C).
- 12.Gently open the blades of the speculum and look for the cervix. It may be necessary to move the speculum up or down until the entire cervix is visible.To reduce patient discomfort and visualize the cervix (WHO [259], C).
- 13.Using the cervix brush, insert the central bristles into the endocervical canal so that the shorter, outer bristles fully contact the ectocervix.
- 14.Using pencil pressure, rotate the brush in a clockwise direction five times. Note: the plastic fronds of the brush are bevelled for rotation in a clockwise direction only.To ensure good contact with the ectocervix and gather a high cellular yield (NHSCSP [161], C).Firm pressure is required to ensure the cells cling to the brush (NHSCSP [161], C).Either:If using ThinPrep, using a swirling motion, rinse the brush into the fixative vial; then push the brush into the base of the vial at least 10 times, forcing the bristles apart.To ensure a usable amount of cellular material is collected (NHSCSP [161], C).Inspect the brush for any residual material and remove any remaining material by passing the brush over the edge of the fixative vial.To ensure the cellular material reaches the preservative solution (NHSCSP [161], C).Ensure that the material reaches the liquid. Then tighten the cap so that the material passes the torque line on the vial and give the vial a shake.To ensure that the cells do not cling to the device (NHSCSP [161], C).Or:If using SurePath, remove the head of the brush from the stem and place into the vial of fixative. Then screw the lid on tightly and shake the vial. Note: it is essential that the sample is placed into the vial immediately in order to achieve fixation.To ensure accurate preservation of cervical material (NHSCSP [161], C).
- 15.Gently pull the speculum out until the blades are clear of the cervix, then close the blades and remove the speculum, placing it in a clinical waste bin if disposable or into a CSS container if reusable.To prevent pinching the cervix or vaginal walls and to ensure safe disposal of contaminated equipment (NHSCSP [161], C).
- 16.Cover the patient and offer tissue paper to wipe away any excess vaginal discharge.To ensure dignity and privacy while promoting hygiene and comfort. E
Post‐procedure
- 17.Remove gloves and dispose of waste in clinical waste receiver.To safely dispose of clinical waste (DH [48], C).
- 18.Assist the patient off the examination table and allow her to dress.To ensure safety and promote dignity and privacy. E
- 19.Using a black ballpoint pen, label the vial with the patient's name, clinic number and date of birth.To ensure the patient's details are documented correctly. E
- 20.Place the vial into a plastic specimen bag with the correctly labelled specimen form and send it to the laboratory.
- 21.Document the procedure in the patient's records.To ensure timely and accurate record keeping (NMC [165], C).