Chapter 13: Diagnostic tests
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Pre‐procedural considerations
Equipment
Commercially available transport media offer a cheap and effective method to enable the culture of both aerobic and anaerobic micro‐organisms. Wound swabbing most frequently involves the use of a cotton‐ or alginate‐tipped swab, although these have been found to inhibit the detection of certain bacteria (Faoagali [60]). Despite being more expensive, flocked nylon swabs now provide a sensitive collection method for culture, rapid and near‐patient testing, and molecular detection of a variety of bacteria and viruses. This is because of their ability to absorb cells and then release them effectively to increase the sensitivity of detection of infecting microbes (Faoagali [60]). If unsure, the practitioner should liaise with the microbiology laboratory to clarify which is the most suitable swab for a particular investigation or type of specimen.
Specific patient preparation
It may be necessary to position the patient in order to obtain the required sample.
Procedure guideline 13.12
Swab sampling: ear
Procedure guideline 13.13
Swab sampling: eye
Procedure guideline 13.14
Swab sampling: nose
Procedure guideline 13.15
Swab sampling: penis
Procedure guideline 13.16
Swab sampling: rectum
Procedure guideline 13.17
Swab sampling: skin
Procedure guideline 13.18
Swab sampling: throat
Procedure guideline 13.19
Swab sampling: vagina
Procedure guideline 13.20