Chapter 20: Diagnostic investigations
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20.1 Cervical uterine smear using liquid‐based cytology
Essential equipment
- Alcoholic handrub
- Light source
- An examination couch
- Disposable clean paper couch covers
- Various sized specula (disposable or reusable)
- Disposable clean gloves
- Cervix brush
- LBC container
- Black ballpoint pen for labelling LBC container
- Specimen form and plastic specimen bag
- A container of warm water
- Box of tissue paper
- Clinical waste container
- Trolley
- Disposable apron
- Lubricant
Optional equipment
- A collecting container for Clinical Sterile Services
Pre‐procedure
ActionRationale
- 1.Discuss benefits and limitations of screening and significance of smear results. Explain the purpose of the procedure and explain what will occur at each step.
- 2.Document the woman's clinical and screening history, specifically:
- date of last menstruation
- any abnormal bleeding
- any unusual vaginal discharge
- contraception
- date of last cervical smear
- any abnormal smear results
- any treatment to the cervix.
- 3.Place all the equipment required for the procedure on the trolley.To facilitate the efficient taking of the smear. E
- 4.Close room door or curtains and ask the patient/client to remove her underwear.To provide privacy and comfort for the patient/client. E
Procedure
- 5.Turn the light source on and position at end of examination table.
- 6.Assist the patient/client into a supine position on the couch, with knees drawn up and legs parted. Keep her as covered as possible.
- 7.Wash hands with antibacterial detergent and running hand‐hot water. Ensure hands are dried with disposable paper hand towels.
- 8.Apply gloves and apron.
- 9.Select the appropriate size speculum, from very small, small, medium, large including a long‐bladed narrow speculum if the vagina is long or the cervix is lying posterior. If a reusable sterilized speculum is used it can be warmed or cooled using clean tap water.
- 10.Apply lubricant to the speculum. Part the labia and, holding the speculum blades together sideways, slip the speculum into the vagina.
- 11.When the speculum is halfway into the vagina, turn it so that the handle is facing down.
- 12.Gently open the blades of the speculum and look for the cervix. It may be necessary to move the speculum up or down until the entire cervix is visible.
- 13.Using the cervix brush, insert the central bristles into the endocervical canal so that the shorter, outer bristles fully contact the ectocervix.
- 14.Using pencil pressure, rotate the brush in a clockwise direction five times and then remove the brush. Note: the plastic fronds of the brush are bevelled for rotation in a clockwise direction only.
- 15.Gently pull the speculum out until the blades are clear of the cervix, then close blades and remove speculum, placing in clinical waste bin if disposable or into CSS container if reusable.
- 16.Cover the patient/client and offer tissue paper to wipe away any excess vaginal discharge.To ensure dignity and privacy while promoting hygiene and comfort. E
Either:
Using Thinprep, using a swirling motion, rinse the brush into the fixative vial; then push the brush into the base of the vial at least 10 times, forcing the bristles apart.
Inspect the brush for any residual material and remove any remaining material by passing the brush over the edge of the fixative vial.
To ensure the cellular material reaches the preservative solution (NHSCSP [102], C; NHSCSP [105], C).
Ensure that the material reaches the liquid. Then tighten the cap so that the material passes the torque line on the vial and give the vial a shake.
Or:
Using SurePath, remove head of the brush from the stem and place into the vial of fixative. Then screw lid on tightly and shake the vial. Note: it is essential that the sample is placed into the vial immediately in order to achieve fixation.
Post‐procedure
- 17.Remove gloves and dispose of waste in clinical waste receiver.
- 18.Assist the patient/client off the examination table and allow her to dress.To ensure safety and promote dignity and privacy. E
- 19.Using a black ballpoint pen, label the vial with patient/client name, clinic number and date of birth.To ensure patient details are documented correctly. E
- 20.Place the vial into plastic specimen bag with the correctly labelled specimen form and send to laboratory.
- 21.Document the procedure in the patient's records.